Computational studies with ESTs: assembly, SNP detection, and applications in alternative splicing
نویسندگان
چکیده
CHAPTER 1 GENERAL INTRODUCTION 1
منابع مشابه
Computational analysis of alternative splicing using EST tissue information.
Expressed sequence tags (ESTs) from normal and tumor tissues have been deposited in public databases. These ESTs and all mRNA sequences were aligned with the human genome sequence using LEADS, Compugen's alternative splicing modeling platform. We developed a novel computational approach to analyze tissue information of aligned ESTs in order to identify cancer-specific alternative splicing and g...
متن کاملA novel algorithm for computational identification of contaminated EST libraries.
A key goal of the Human Genome Project was to understand the complete set of human proteins, the proteome. Since the genome sequence by itself is not sufficient for predicting new genes and alternative splicing events that lead to new proteins, expressed sequence tags (ESTs) are used as the primary tool for these purposes. The high prevalence of artifacts in dbEST, however, often leads to inval...
متن کاملA novel algorithm for computational identi®cation of contaminated EST libraries
A key goal of the Human Genome Project was to understand the complete set of human proteins, the proteome. Since the genome sequence by itself is not suf®cient for predicting new genes and alternative splicing events that lead to new proteins, expressed sequence tags (ESTs) are used as the primary tool for these purposes. The high prevalence of artifacts in dbEST, however, often leads to invali...
متن کاملNovel peptide identification from tandem mass spectra using ESTs and sequence database compression
Peptide identification by tandem mass spectrometry is the dominant proteomics workflow for protein characterization in complex samples. Traditional search engines, which match peptide sequences with tandem mass spectra to identify the samples' proteins, use protein sequence databases to suggest peptide candidates for consideration. Although the acquisition of tandem mass spectra is not biased t...
متن کاملDetection and prediction of alternative splicing in Arabidopsis thaliana
Alternative splicing is an important process for increasing the diversity arising from a single gene. Presently, most studies aimed at detecting alternatively spliced genes use Expressed Sequence Tags (ESTs). However, the EST studies based on spliced transcripts analyse sequences by alignment rather than sequence patterns. Second, EST libraries can be of uncertain quality. To address these issu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2015